Bachelor of Arts
Biological and Physical Sciences
Program or Major
Understanding the metabolic pathway of a drug is an integral part of pharmaceutical development. Microsomal metabolism is the industry standard to model the metabolism of the drug by the liver in vitro. Our studies employ carbamazepine (CBZ) as a CYP3A4 substrate to develop a method to investigate potential drug-drug interactions involving this enzyme. Toward this end, the metabolite of CBZ, carbamazepine-10,11-epoxide (CBZ-E), was synthesized for use as a standard. The purity of the synthesized CBZ-E was 95.2%, and the percent yield was 76.16%. The use of human liver microsomes is a common incubation method used to model drug metabolism in an in vitro setting. At this time, we have begun to develop techniques for analyzing the human metabolism of CBZ with a view toward using this pathway to probe drug-drug interactions. However, we have yet to perform a completely successful metabolism of CBZ. Potential problems with our method may be in the HPLC analysis or in the low concentration of microsomal protein. Efforts on this project continue.
Iacoviello, Sarah, "Development of Drug-Drug Interaction Research Techniques Involving the CYP3A4 Human Liver Enzyme" (2023). Honors Theses. 119.